GK027 ScienCell 人類非經(jīng)典Wnt信號(hào)通路qPCR檢測試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室（ScienCell）是一家生物技術(shù)公司，其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實(shí)現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進(jìn)口，貨期穩(wěn)定，折扣好的服務(wù)標(biāo)準(zhǔn)。

GK027 ScienCell人類非經(jīng)典Wnt信號(hào)通路qPCR檢測試劑盒，Human Noncanonical Wnt Signaling Pathways qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌; ScienCell

產(chǎn)品貨號(hào)：GK027

產(chǎn)品名稱：GK027 ScienCell人類非經(jīng)典Wnt信號(hào)通路qPCR檢測試劑盒，Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK027 ScienCell人類非經(jīng)典Wnt信號(hào)通路qPCR檢測試劑盒，Human Noncanonical Wnt Signaling Pathways qPCR Array Kit， 產(chǎn)品說明：

ScienCell 的 GeneQuery™ 人類非規(guī)范 Wnt 信號(hào)通路 qPCR 陣列試劑盒 （GQH-WAP） 旨在促進(jìn)參與替代 Wnt 通路的 88 個(gè)關(guān)鍵基因的基因表達(dá)譜分析。 該試劑盒強(qiáng)調(diào)參與平面細(xì)胞極性信號(hào)傳導(dǎo)和鈣信號(hào)轉(zhuǎn)導(dǎo)的基因。 如何根據(jù)功能對(duì)所包含的基因進(jìn)行分組的簡要示例如下所示：

平面電池極性：DAAM1、CELSR1、FAT1、FZD7、VANGL1

鈣： 鈣， 冷靜1， 磷脂1， FZD4， NFATC1

信令調(diào)制器： LRP5， LRP6， SPRY1， EFNB， CASR

受體：WNT2B、WNT4、WNT5A、WNT9A、WNT11

靶基因：RHOA，RACGAP1，CREBBP，CFL1，FUZ

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個(gè)孔包含一個(gè)引物組，可識(shí)別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計(jì)的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery™ Human Noncanonical Wnt Signaling Pathways qPCR Array Kit (GQH-WAP) is designed to facilitate gene expression profiling of 88 key genes involved in alternative Wnt pathways.  This kit emphasizes genes involved in planar cell polarity signaling and calcium signal transduction.  Brief examples of how included genes may be grouped according to function are shown below:

·         Planar cell polarity: DAAM1, CELSR1, FAT1, FZD7, VANGL1

·         Calcium: CASR, CALM1, FOSL1, FZD4, NFATC1

·         Signaling modulator: LRP5, LRP6, SPRY1, EFNB, CASR

·         Receptors: WNT2B, WNT4, WNT5A, WNT9A, WNT11

·         Target genes: RHOA, RACGAP1, CREBBP, CFL1, FUZ

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

基因查詢™人類非規(guī)范 Wnt 信號(hào)通路 qPCR 陣列板布局* （8 個(gè)粗體和斜體對(duì)照）

提示：基因選擇可能會(huì)根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息：

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