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ScienCellGK063人細胞周期蛋白依賴性激酶檢測試劑盒
更新時間:2023-01-03   點擊次數(shù):499次

GK063 ScienCell 人細胞周期蛋白和細胞周期蛋白依賴性激酶qPCR檢測試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNAcDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務(wù)標準。

GK063 ScienCell人細胞周期蛋白和細胞周期蛋白依賴性激酶qPCR檢測試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK063

產(chǎn)品名稱:GK063 ScienCell人細胞周期蛋白和細胞周期蛋白依賴性激酶qPCR檢測試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

GK063 ScienCell人細胞周期蛋白和細胞周期蛋白依賴性激酶qPCR檢測試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit 產(chǎn)品說明:

ScienCell GeneQuery™ 人細胞周期蛋白和細胞周期蛋白依賴性激酶 qPCR 陣列試劑盒 (GQH-CCN) 旨在促進 40 種選定細胞周期蛋白和細胞周期蛋白依賴性激酶 (CDK) 的基因表達譜分析。CDK是細胞周期進程和細胞分裂所需的一類小酶,通過其底物的磷酸化。CDK必須與細胞周期蛋白結(jié)合才能激活。細胞周期蛋白/CDK復(fù)合物調(diào)節(jié)細胞周期檢查點,是細胞周期進程的驅(qū)動力。下面顯示了如何對所包含的基因進行分組的簡要示例:

細胞周期蛋白: CCNA CCNB CCNC CCND CCNE CCNF CCNG CCNH CCNI CCNK CCNL CCNO CCNT CCNY

CDK CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK10 CDK20

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設(shè)計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit (GQH-CCN) is designed to facilitate gene expression profiling of 40 selected cyclins and cyclin-dependent kinases (CDKs). CDKs are a family of small enzymes required for cell cycle progression and cell division, by means of phosphorylation of their substrates. CDKs must bind to cyclins to become fully activated. Cyclin/CDK complexes regulate cell cycle checkpoints and are the driving force of cell cycle progression. Brief examples of how included genes may be grouped are shown below:

·         Cyclins: CCNA, CCNB, CCNC, CCND, CCNE, CCNF, CCNG, CCNH, CCNI, CCNK, CCNL, CCNO, CCNT, CCNY

·         CDKs: CDK1, CDK2, CDK3, CDK4, CDK5, CDK6, CDK7, CDK8, CDK10, CDK20

GeneQuery qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人細胞周期蛋白和細胞周期蛋白依賴性激酶qPCR陣列板布局*8 個粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

CCNA1

CCND3

CCNI2

CDK2

CDK11A

β-actin

CCNA1

CCND3

CCNI2

CDK2

CDK11A

β-actin

B

CCNA2

CCNE1

CCNK

CDK3

CDK12

GAPDH

CCNA2

CCNE1

CCNK

CDK3

CDK12

GAPDH

C

CCNB1

CCNE2

CCNL1

CDK4

CDK13

LDHA

CCNB1

CCNE2

CCNL1

CDK4

CDK13

LDHA

D

CCNB2

CCNF

CCNL2

CDK5

CDK15

NONO

CCNB2

CCNF

CCNL2

CDK5

CDK15

NONO

E

CCNB3

CCNG1

CCNO

CDK6

CDK16

PPIH

CCNB3

CCNG1

CCNO

CDK6

CDK16

PPIH

F

CCNC

CCNG2

CCNT1

CDK7

CDK17

GDC

CCNC

CCNG2

CCNT1

CDK7

CDK17

GDC

G

CCND1

CCNH

CCNY

CDK8

CDK19

PPC

CCND1

CCNH

CCNY

CDK8

CDK19

PPC

H

CCND2

CCNI

CDK1

CDK10

CDK20

NTC

CCND2

CCNI

CDK1

CDK10

CDK20

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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