GK090 ScienCell 人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit
ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。
北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。
GK090 ScienCell人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit,產(chǎn)品簡介:
產(chǎn)品品牌: ScienCell
產(chǎn)品貨號:GK090
產(chǎn)品名稱:GK090 ScienCell人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit
產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well
GK090 ScienCell人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit, 產(chǎn)品說明:
ScienCell 的 GeneQuery™ 人類細胞連接 qPCR 陣列試劑盒 (GQH-JXN) 分析了參與細胞連接形成、組織和維護的 88 個關鍵基因。 該試劑盒可研究對細胞通訊、細胞旁轉(zhuǎn)運和錨定很重要的細胞-細胞連接。 如何對所包含的基因進行分類的簡要示例如下所示:
間隙連接點: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1
粘附連接: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2
緊密結: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)
橋粒: DSC1, DSG1, DSP, ITGB4, JUP
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(i)qPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有說明,否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;(iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。
ScienCell's GeneQuery™ Human Cell Junctions qPCR Array Kit (GQH-JXN) profiles 88 key genes involved in cell junction formation, organization, and maintenance. This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring. Brief examples of how included genes may be categorized are shown below:
· Gap junctions: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1
· Adherens junctions: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2
· Tight junctions: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)
· Desmosomes: DSC1, DSG1, DSP, ITGB4, JUP
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
基因查詢™人類細胞連接 qPCR 陣列板布局*(8 個粗體和斜體對照)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | ACTA1 | CADM1 | CDH19 | CELSR3 | CLDN8 | CXADR | DSP | GJA5 | JAM3 | NECTIN3 | PECAM1 | β-actin |
B | ACTA2 | CADM3 | CDH2 | CGN | CLTA | DCHS1 | F11R | GJB1 | JUP | OCLN | PVR | GAPDH |
C | ACTB | CDH1 | CDH3 | CLDN1 | CLTB | DCHS2 | FAT1 | GJB6 | LEF1 | PAK1 | RHOA | LDHA |
D | ACTC1 | CDH10 | CDH5 | CLDN10 | CLTC | DNM1 | FAT2 | ICAM1 | MAGI1 | PANX1 | SNAI1 | NONO |
E | ACTG1 | CDH11 | CDX1 | CLDN2 | CLTCL1 | DSC1 | FAT3 | ILK | MAGI2 | PARD3 | SP1 | PPIH |
F | ACTG2 | CDH13 | CDX2 | CLDN4 | CSNK1D | DSC2 | FAT4 | ITGB1 | MYLK | PARD6A | SRC | GDC |
G | ANG | CDH15 | CELSR1 | CLDN5 | CTNNB1 | DSG1 | GJA1 | ITGB4 | NECTIN1 | PCDH7 | TJP1 | PPC |
H | AP2M1 | CDH16 | CELSR2 | CLDN7 | CTNND1 | DSG3 | GJA4 | JAM2 | NECTIN2 | PCDH8 | WNK4 | NTC |
提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新
ScienCell基因表達分析產(chǎn)品訂購信息:
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