GK091 ScienCell 人肝星狀細胞生物學qPCR檢測試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit
ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產品的研究和發展。 ScienCell為研究界提供各種高質量的正常的人類和動物細胞,細胞培養基和試劑,培養基添加因子,細胞來源的RNA,cDNA和蛋白質。ScienCell的科學家們以改善生活質量,實現細胞的能力,打擊人類退化性疾病為目的研究和開發各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。
北京諾博萊德作為ScienCell品牌的經銷商,為客戶提供,正規進口,貨期穩定,折扣好的服務標準。
GK091 ScienCell人肝星狀細胞生物學qPCR檢測試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit,產品簡介:
產品品牌: ScienCell
產品貨號:GK091
產品名稱:GK091 ScienCell人肝星狀細胞生物學qPCR檢測試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit
產品規格:One 96-well plate, one 20ul reaction per well
GK091 ScienCell人肝星狀細胞生物學qPCR檢測試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit, 產品說明:
ScienCell 的 GeneQuery™ 人肝星狀細胞生物學 qPCR 芯片試劑盒 (GQH-HSB) 旨在促進 88 個關鍵基因的基因表達譜分析,這些基因涉及 (i) 人肝星狀細胞活化和增殖;(二)視黃醇代謝;(iii)細胞外基質(ECM)合成和重塑;以及(iv)肝星狀細胞相關疾病。如何根據其功能對所包含的基因進行分組的簡要示例如下所示:
肝星狀細胞標志物:ACTA2,GFAP,DES,NGFR,COL2A1,VIM,LAMA1,NES
肝星狀細胞活化和增殖:ICAM1,MMP13,RELN,TIMP1,SYP,CDH1,RARA,PDGF,TGFB1,MMP2
肝臟發育和再生:STX2,PTN,SYNM,FOXF1,ICAM1,VCAM1,ITGB3,HGF,CXCL12,DES
視黃醇代謝: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
ECM合成和重塑:TGFB1,COL4A2,層粘連蛋白亞基,SPARC,VCAN,CTGF
細胞因子生產:EGF,IGF,PDGF,FGF,CSF1,CCL2,TNF,CXCL2,CXCL3,CXCL8
肝纖維化和癌癥發展:PKHD1,TMEM67,RPGRIP1L,SLC17A5,PKD1,CC2D2A,B9D1,B9D2
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設計的引物確保:(i)qPCR分析中的優良退火溫度為65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有說明,否則引物組可識別靶基因的所有已知轉錄本變體;(iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。
ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:
· Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES
· Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2
· Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES
· Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
· ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
· Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8
· Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
基因查詢™人肝星狀細胞生物學qPCR陣列板布局*(8 個粗體和斜體控件)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | ACTA2 | COL2A1 | CXCL2 | FGF1 | IGF1 | LAMB1 | MYH10 | NGFR | RARA | SPARC | TIMP1 | β-actin |
B | B9D1 | COL4A2 | CXCL3 | FGF2 | IGF2 | LAMB3 | MYH11 | PDGFA | RBP1 | SPP1 | TMEM67 | GAPDH |
C | B9D2 | CRABP1 | CXCL8 | FOXF1 | IL1A | LAMC1 | MYH9 | PDGFB | RBP2 | STX2 | TNC | LDHA |
D | BDNF | CRABP2 | CYP26A1 | GFAP | ITGB3 | LEP | NCAM1 | PDGFC | RBP4 | SYNM | TNF | NONO |
E | CC2D2A | CSF1 | CYP26B1 | HAS1 | KLF6 | LHX2 | NCAM2 | PDGFD | RBP5 | SYP | VCAM1 | PPIH |
F | CCL2 | CTGF | DES | HGF | LAMA1 | LRAT | NES | PKD1 | RELN | TGFB1 | VCAN | GDC |
G | CDH1 | CXCL10 | EGF | ICAM1 | LAMA2 | MMP13 | NFKB1 | PKHD1 | RPGRIP1L | TGFBI | VIM | PPC |
H | CLEC3B | CXCL12 | F2 | IFNG | LAMA3 | MMP2 | NFKB2 | PTN | SLC17A5 | THBS2 | VTN | NTC |
提示:基因選擇可能會根據新的研究和開發進行更新
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