GK118 ScienCell 人類先天淋巴細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit
ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。
北京諾博萊德作為ScienCell品牌的特約經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。
GK118 ScienCell人類先天淋巴細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit,產(chǎn)品簡(jiǎn)介:
產(chǎn)品品牌; ScienCell
產(chǎn)品貨號(hào):GK118
產(chǎn)品名稱:GK118 ScienCell人類先天淋巴細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit
產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well
GK118 ScienCell人類先天淋巴細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit, 產(chǎn)品說明:
ScienCell 的 GeneQuery™ 人類先天淋巴細(xì)胞發(fā)育標(biāo)記 qPCR 陣列試劑盒 (GQH-ILC) 旨在促進(jìn)參與常見淋巴祖細(xì)胞 (CLP) 分化為先天淋巴細(xì)胞 (ILC)、自然殺傷 (NK) 細(xì)胞和淋巴組織誘導(dǎo) (LTi) 細(xì)胞的 24 個(gè)標(biāo)記基因的基因表達(dá)譜。如何根據(jù)其功能對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:
CLP 標(biāo)記物: MME, CD34, IL7R, FLT3
未成熟的NK細(xì)胞標(biāo)志物:CD33,PROM1,CD34,KIT,IL2RB,KLRK1
成熟的NK細(xì)胞標(biāo)志物:FCGR3A,IL2RA,NCAM1,B3GAT1,KLRD1,KLRC1,NCR1 / 2 / 3
ILC祖細(xì)胞標(biāo)志物:CD34,KIT,IL7R
ILC 標(biāo)記:NCAM1、KLRD1、ITGAE、IL7R、KLRB1、PTPRC、NCR1、RORC
LTi 細(xì)胞標(biāo)志物:CD4、IL7R、CCR6
注意:所有基因名稱都遵循人類基因組組織基因命名委員會(huì)(HGNC)的guanfang符號(hào)。
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可以特異性識(shí)別并有效擴(kuò)增靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(i)qPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有說明,否則引物組可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;(iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。
ScienCell's GeneQuery™ Human Innate Lymphoid Cell Development Markers qPCR Array Kit (GQH-ILC) is designed to facilitate gene expression profiling of 24 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into innate lymphoid cells (ILCs), natural killer (NK) cells, and lymphoid tissue inducer (LTi) cells. Brief examples of how included genes may be grouped according to their functions are shown below:
· CLP markers: MME, CD34, IL7R, FLT3
· Immature NK cell markers: CD33, PROM1, CD34, KIT, IL2RB, KLRK1
· Mature NK cell markers: FCGR3A, IL2RA, NCAM1, B3GAT1, KLRD1, KLRC1, NCR1/2/3
· ILC progenitor markers: CD34, KIT, IL7R
· ILC markers: NCAM1, KLRD1, ITGAE, IL7R, KLRB1, PTPRC, NCR1, RORC
· LTi cell markers: CD4, IL7R, CCR6
Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
基因查詢™ 人類先天淋巴細(xì)胞發(fā)育標(biāo)志物 qPCR 陣列板布局*(8 個(gè)粗體和斜體控件)
注意:所有基因名稱都遵循HGNC的guanfang符號(hào)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | B3GAT1 | IL2RB | MME | ACTB | B3GAT1 | IL2RB | MME | ACTB | B3GAT1 | IL2RB | MME | ACTB |
B | CCR6 | IL7R | NCAM1 | GAPDH | CCR6 | IL7R | NCAM1 | GAPDH | CCR6 | IL7R | NCAM1 | GAPDH |
C | CD33 | ITGAE | NCR1 | LDHA | CD33 | ITGAE | NCR1 | LDHA | CD33 | ITGAE | NCR1 | LDHA |
D | CD34 | KIT | NCR2 | NONO | CD34 | KIT | NCR2 | NONO | CD34 | KIT | NCR2 | NONO |
E | CD4 | KLRB1 | NCR3 | PPIH | CD4 | KLRB1 | NCR3 | PPIH | CD4 | KLRB1 | NCR3 | PPIH |
F | FCGR3A | KLRC1 | PROM1 | GDC | FCGR3A | KLRC1 | PROM1 | GDC | FCGR3A | KLRC1 | PROM1 | GDC |
G | FLT3 | KLRD1 | PTPRC | PPC | FLT3 | KLRD1 | PTPRC | PPC | FLT3 | KLRD1 | PTPRC | PPC |
H | IL2RA | KLRK1 | RORC | NTC | IL2RA | KLRK1 | RORC | NTC | IL2RA | KLRK1 | RORC | NTC |
提示:基因選擇可能會(huì)根據(jù)新的研究和開發(fā)進(jìn)行更新
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